Journal of Andrology Testis Workshop 2009
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Published-Ahead-of-Print May 28, 2009, DOI:10.2164/jandrol.108.007401

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Identification of the Major Proteins Produced by Cultured Germline Stem Cell in Chicken

Beom K Han , Jin G Jung , Jungrye Nam , Jong K Moon , Jin N Kim , Sang I Lee , Jin-Kyoo Kim , Heebal Kim , and Jae Y Han *

* To whom correspondence should be addressed. E-mail: jaehan{at}snu.ac.kr.

Although chicken spermatogonial stem cells (SCs) are important in spermatogenesis and transgenic research, little is known about these cells. Recently, our group constructed an in vitro culture system to establish germline stem cells (GSCs). To examine the mechanism of chicken spermatogonial SCs development, we constructed a proteome map of GSCs from 4-week-old chicken testes. Soluble extracts of the GSCs were fractionated by two-dimensional gel electrophoresis (pH 4-7). Several protein spots, including those that displayed significantly high levels, were identified by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry and LC-MS/MS. Of the 82/250 GSCs spots examined, 56 yielded mass spectra that matched avian proteins found in the on-line databases. All of the identified proteins were classified into functional groups. This type of proteome map is an important resource for research on spermatogenesis and transgenesis.


Key words: Reproductive Genetics • Spermatogenesis • chicken • germline stem cell • mass spectrometry • spermatogonial stem cell






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