Journal of Andrology Testis Workshop 2009
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Published-Ahead-of-Print January 8, 2009, DOI:10.2164/jandrol.108.006775

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Immunolocalization of Androgen, Estrogen alpha and beta Receptors in Experimentally Induced Canine Prostatic Hyperplasia

Fanny Gallardo , Josep Lloreta , Félix García , Xavier Moll , Teresa Baró , Luciano A. González , Joan Morote , Jaume Reventós , and Teresa Mogas *

* To whom correspondence should be addressed. E-mail: teresa.mogas{at}uab.es.

Benign prostatic hyperplasia (BPH) is an age-dependent prostatic disease affecting male humans and dogs. In dogs, the combined administration of estrogens and androgens synergistically increases prostate weight, and continued treatment leads to the development of glandular hyperplasia. The aim of the present study was to examine the immunohistochemical expression of androgen (AR), estrogen {alpha} (ER{alpha}) and {beta} (ER{beta}) receptors in the different cell types of the prostate gland in a model of experimentally. Five male Beagle dogs were castrated and treated with 25 mg of 5{alpha} -androstane-3{alpha}, 17{beta}-diol and 0.25 mg 17{beta}-estradiol for 30 weeks. Prostate specimens were surgically obtained every 45 days (experimental stages M0 to M6). The control group consisted of three non-castrated dogs treated with vehicle, from which specimens were only taken at the time points M0, M1, M4 and M6. Immunohistochemical data revealed high AR and ER{alpha} expression in the epithelial and stromal cell nuclei of all the experimental and control specimens. Weak staining of the cytoplasm was only observed in epithelial cells. The suspension of hormone treatment led to a significant reduction in the expression of both receptors. On the contrary, ER{beta} was only expressed in epithelial cell nuclei, with no significant differences in the percentages of stained nuclei between control and hormonally treated or atrophic prostates. Results indicate that AR, ER{alpha}, and ER{beta} are differently expressed in canine prostate tissue and that they show specific expression patterns in response to the hormonal induction of BPH.


Key words: Androgen • Hormone • Prostate • Animal model • Dog • Prostatic hyperplasia






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