Journal of Andrology
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Published-Ahead-of-Print July 3, 2008, DOI:10.2164/jandrol.108.005819

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Trevor G. Cooper
Barbara Hellenkemper
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Method-Related Estimates of Sperm Vitality

Trevor G. Cooper * and Barbara Hellenkemper

* To whom correspondence should be addressed. E-mail: trevorg.cooper{at}ukmuenster.de.

Comparison of methods that estimate viability of human spermatozoa by monitoring head membrane permeability revealed that wet preparations (whether using positive or negative phase contrast microscopy) generated significantly higher percentages of non-viable cells than air-dried, eosin-nigrosin smears. Only with the latter method did the sum of motile (presumed live) and stained (presumed dead) preparations never exceed 100%, making this the method of choice for sperm viability estimates.


Key words: Semen Analysis • Sperm • optics • technique






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