Journal of Andrology Testis Workshop 2009
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Published-Ahead-of-Print October 30, 2008, DOI:10.2164/jandrol.108.005629

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Procyanidin Induces Apoptosis and Necrosis of Prostate Cancer Cell Line PC-3 in Mitochondrion-Dependent Manner

Xuejun Shang *, Genhong Yao , Jingping Ge , Yi Sun , WH Teng , and Yufeng Huang

* To whom correspondence should be addressed. E-mail: shangxj{at}androl.cn.

Our objective was to study the effects of procyanidin on the cell death of human hormone-resistant prostate carcinoma cell line PC-3 and its mechanism. PC-3 cells were treated with procyanidin of different concentrations. The cell apoptosis rates were detected by Annexin V-FITC and propidium iodide (PI) double stainin followed by Flow cytometry (FCM) analysis. Mitochondrial membrane potential was analyzed by FCM with Rhodamine 123 (Rh123) staining. After 24h treatment with 300 µg/ml procyanidin, the apoptosis rate of PC-3 cells was 44.86%, and {Delta}{Psi}m was significantly decreased by 87.30%. With the extending of procyanidin treatment, the apoptosis rate decreased while the necrosis rate increased. Procyanidin could induce apoptosis and necrosis in PC-3 cells, which might be related with down-regulation of mitochondrial membrane {Delta}{Psi}m.


Key words: PC-3 cell • apoptosis • mitochondrial membrane potential • necrosis • procyanidin






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