Journal of Andrology
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Published-Ahead-of-Print October 16, 2008, DOI:10.2164/jandrol.108.004929

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Paraoxonase-1 Activity in Subfertile Men and Relationship to Sperm Parameters

Fatma Ferda Verit *, Ayhan Verit , Halil Ciftci , Ozcan Erel , and Hakim Celik

* To whom correspondence should be addressed. E-mail: fverit{at}harran.edu.tr.

Oxidative stress has been implicated in the pathogenesis of male infertility. Paraoxonase-1 (PON-1) is a high density lipoprotein associated antioxidant enzyme that prevents oxidative modification of low density lipoprotein. Our aims of the study were to investigate i) Seminal PON-1 activity in subfertile men ii) whether it had any relationship with semen parameters. The study included 28 idiopathic subfertile, 32 subfertile male with abnormal semen parameters and 30 fertile volunteers. Seminal PON-1 activity was measured spectrophotometrically. Seminal total antioxidant status (TAS) and total oxidant status (TOS) were determined by using colorimetric methods. Oxidative stress index (OSI) was calculated as [(TOS/TAS) x100]. TOS and OSI were significantly higher and PON-1 activity and TAS were significantly lower in subfertile men than in idiopathic subfertile men and fertile donors. PON-1 activity was also strongly correlated with sperm concentration (r=0.68, p<0.0001), motility (r=0.58, p<0.0001) and morphology (r=0.62, p<0.0001) in overall group. The receiver operating characteristic curve (ROC) analysis revealed a high diagnostic value for PON-1 activity with respect to male factor subfertility, with an area under curve of 0.95 (95% confidence interval (CI) =0.89-1.01), sensitivity = 97% and specificity = 88%. Men with abnormal semen parameters have decreased levels of PON-1 activity in their seminal plasma. This may play an important role in the pathogenesis of male factor subfertility.


Key words: Infertility • PON-1 activity • oxidative stress • spermatozoa




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L. A. Lazaros, N. V. Xita, E. G. Hatzi, A. I. Kaponis, T. J. Stefos, N. I. Plachouras, G. V. Makrydimas, N. V. Sofikitis, K. A. Zikopoulos, and I. A. Georgiou
Association of Paraoxonase Gene Polymorphisms With Sperm Parameters
J Androl, July 1, 2011; 32(4): 394 - 401.
[Abstract] [Full Text] [PDF]


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