Journal of Andrology
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Published-Ahead-of-Print April 25, 2007, DOI:10.2164/jandrol.107.002725

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Cryo-survival and in vitro fertilizing capacity post-thaw is improved when boar spermatozoa are frozen in the presence of seminal plasma from good freezer boars

Marta Hernández , Jordi Roca *, Juan J Calvete , Libia Sanz , Teresa Muiño-Blanco , José A. Cebrián-Pérez , Juan M. Vázquez , and Emilio A. Martínez

* To whom correspondence should be addressed. E-mail: roca{at}um.es.

The study evaluated the protective effect of seminal plasma (SP) added to freezing extender, against cryopreservation injuries to boar spermatozoa. Pooled sperm-rich fractions collected from 9 fertile boars were frozen in 0.5 mL straws, after being extended in a conventional freezing extender either alone or supplemented with 5% of SPs (SP1-SP4), collected from the sperm rich fractions (diluted 1:1, v/v, in BTS extender) from four boars (1-4) with known sperm cryosurvival (poor, moderate and good sperm freezers). Cryopreservation injuries were assessed in terms of post-thaw sperm motility (assessed by CASA), viability (plasma membrane and acrosome integrity assessed simultaneously by flow cytometry), membrane lipid peroxidation (MDA production) and the ability of thawed spermatozoa to fertilize in vitro matured homologous oocytes. The addition of SP from "good" sperm freezers (SP3 and 4) improved (P < .01) the motility and viability of thawed spermatozoa, without any influence on MDA production. Moreover, SP from "good" sperm freezers also increased (P < .05) the percentage of penetrated (SP3) and polyspermic oocytes (SP4) respect to the control. Neither the total amount of SP proteins, protein profiles, nor antioxidant capacity of the different SPs were related to the various cryosurvival/fertilizing capacity of the processed spermatozoa


Key words: Assisted reproduction • Cryopreservation • Semen • Semen Analysis • Sperm






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Copyright © 2007 by The American Society of Andrology.