Journal of Andrology
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Published-Ahead-of-Print April 4, 2007, DOI:10.2164/jandrol.106.001271

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Tag STS in the AZF region associated with azoospermia in Tunisian population

lobna hadjkacem-Loukil *, Imen Ayadi , Ali Bahloul , Hammadi Ayadi , and Leila Ammar-Keskes

* To whom correspondence should be addressed. E-mail: lobna_h{at}lycos.com.

The aim of this study was to determine the appropriate tag STS associated with azoospermia. We evaluated the incidence of Y chromosome microdeletions in Tunisian infertile male patients by PCR using 14 Sequence Tagged Sites (STSs) in AZF region of Yq11. A logistic regression analysis was performed in order to test the association of STSs with semen quality. Haploview version 3.11 was used to identify the possible blocks of deletion involving a minimum number of STSs and that can be used to tag the deletion block in future analysis. Using the 14 STSs, 48% infertile patients (102 of 210) had microdeletions of Y chromosome, but, following the European Academy of Andrology (EAA) guidelines, only 16% of patients had microdeletions. Statistically significant difference was found with some STSs for azoospermia and oligozoospermia. A candidate combinaison composed of four STSs (RBMY-sy157-sy84-sy130) was associated with azoospermia in Tunisian population. According to this study, this tag can be used in the screening of Y chromosome microdeletions before assisted reproduction treatment in Tunisian population.


Key words: Infertility • AZF • Haploview • Tag STS • Y chromosome microdeletions • azoospermia






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