Journal of Andrology Testis Workshop 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published-Ahead-of-Print January 24, 2007, DOI:10.2164/jandrol.106.000869

This Article
Right arrow Author Manuscript (PDF)
Right arrow All Versions of this Article:
28/4/493    most recent
Author Manuscript (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, M. W.
Right arrow Articles by Overstreet, J. W
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, M. W.
Right arrow Articles by Overstreet, J. W

Damage to Chromosomes and DNA of Rhesus Monkey Sperm Following Cryopreservation

Ming Wen Li , Stuart Meyers *, Theodore L Tollner , and James W Overstreet

* To whom correspondence should be addressed. E-mail: smeyers{at}ucdavis.edu.

Fresh and frozen-thawed rhesus monkey sperm were analyzed for DNA damage using the comet assay and for chromosome damage by cytogenetic analysis after intracytoplasmic sperm injection (ICSI) into mouse oocytes. The percentage of fresh sperm with damaged DNA in ejaculated semen was 0 to 2.7 % (n=5). Conventional cryopreservation and storage in liquid nitrogen caused DNA damage in 25.3 to 43.7 % of sperm, and when sperm were frozen without cryoprotectants, 52.7 to 92.0 % of thawed sperm had DNA damage. However, no significant difference in chromosome damage was found between fresh sperm and frozen-thawed sperm when motile sperm were selected for ICSI. The percentage of sperm with abnormal karyotypes ranged from 0 to 8.3%. The most common structural chromosomal abnormalities in fresh motile sperm and frozen-thawed motile sperm were chromosome breaks or fragments. Our findings suggest that genetically competent frozen-thawed macaque sperm can be selected for fertilization by using only motile sperm for ICSI.


Key words: Cryopreservation • ICSI • Sperm • Comet assay • DNA damage • nonhuman primate • rhesus




This article has been cited by other articles:


Home page
 J AndrolHome page
Q. Dong, S. E. Rodenburg, C. Huang, and C. A. Vandevoort
Cryopreservation of Rhesus Monkey (Macaca mulatta) Epididymal Spermatozoa Before and After Refrigerated Storage
J Androl, May 1, 2008; 29(3): 283 - 292.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2007 by The American Society of Andrology.