Journal of Andrology
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Published-Ahead-of-Print August 9, 2006, DOI:10.2164/jandrol.106.000174

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Successful pregnancy and delivery following intracytoplasmic injection of frozen-thawed non-viable testicular sperm and oocyte activation with calcium ionophore

Ali Ahmady * and Essam Michael

* To whom correspondence should be addressed. E-mail: ali_ahmady{at}yahoo.com.

This is a case report of successful pregnancy and full term delivery following injection of non-viable frozen-thawed testicular sperm into the cytoplasm of a mature oocyte, from a poor responder patient, followed by oocyte activation with Calcium inophore. In this communication, we have shown that activation of the oocyte is necessary in order to achieve fertilization when non-viable frozen testicular sperm is used for ICSI.


Key words: ICSI • Calcium ionophore • Dead sperm • Oocyte activation • Testicular sperm






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