Journal of Andrology
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Published-Ahead-of-Print September 20, 2006, DOI:10.2164/jandrol.106.000158

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Pregnancies in cryptozoospermia using sperm ejaculated one day before ICSI: four case reports

Isabelle Koscinski *, Stéphane Viville , Valérie Lefebvre-Khalil , André Defossez , and Jean M Rigot

* To whom correspondence should be addressed. E-mail: isabelle.koscinski{at}chru-strasbourg.fr.

BACKGROUND: In cryptozoospermia, the absence of sperm on the day of the intracytoplasmic sperm injection (ICSI) attempt often requires the cryopreservation of sperm or, when this fails, a Testis biopsy. We report here, four cases of cryptozoospermia where the ICSI attempt was performed with sperm ejaculated the previous day. MATERIAL AND METHODS: We failed to create a cryopreserved safety pool of sperm for four cryptozoospermic men with transient azoospermia. Their sperm was studied after 24h culture, and at least 10 motile or living spermatozoa were found. Patients were thus asked to produce an ejaculate on the day before each fertilisation attempt and the sperm were maintained overnight in culture. On the day of oocyte retrieval, no sperm was found in two patients' ejaculates and the cultured spermatozoa were used. RESULTS: Using one-day-old spermatozoa, two of four patient couples had high fertilisation rates (50% and 75%) and achieved pregnancy. In contrast, the fertilisation rates of the two other patients were 27% and 28%. CONCLUSION: Spermatozoa ejaculated one day before fertilization can be used successfully from patients with cryptozoospermia, as an alternative to the recommended cryopreserved safety pool of sperm.


Key words: Assisted reproduction • Infertility • Sperm retrieval • Cryptozoospermia






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