Journal of Andrology
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Published-Ahead-of-Print August 9, 2006, DOI:10.2164/jandrol.106.000067

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Increased Aneuploidy Rate in Sperm with Fragmented DNA as Determined by the Sperm Chromatin Dispersion (SCD) Test and Fish Analysis

Lourdes Muriel , Vicente Goyanes , Enrique Segrelles , Jaime Gosalvez , Juan G. Alvarez , and Jose Luis Fernandez *

* To whom correspondence should be addressed. E-mail: jlfernandez{at}canalejo.org.

Previous studies suggest that sperm DNA fragmentation may be associated with aneuploidy. However, current tests available did not allow to simultaneously perform DNA fragmentation and chromosomal analyses in the same sperm cell. The recently introduced Sperm Chromatin Dispersion (SCD) test allows to determine this relationship. Semen samples from sixteen males, including four fertile donors, seven normozoospermic, three teratozoospermic, one asthenozoospermic and one oligoasthenoteratozoospermic, were processed for DNA fragmentation analysis by the SCD test using the Halosperm kit. Three-color Fluorescence In Situ Hybridization (FISH) was performed on SCD-processed slides to determine aneuploidy for chromosomes X, Y and 18. Spermatozoa with DNA fragmentation showed a 4.4±1.9-fold increase in diploidy rate, and a 5.9±3.5-fold increase in disomy rate compared to spermatozoa without DNA fragmentation. The overall aneuploidy rate was 4.6±2.0-fold higher in sperm with fragmented DNA (Wilcoxon rank test: p<0.001 in the three comparisons). A higher frequency of DNA fragmentation was found in sperm cells containing sex chromosome aneuploidies originated in both first and second meiotic divisions. The observed increase in aneuploidy rate in sperm with fragmented DNA may suggest that the occurrence of aneuploidy during sperm maturation may lead to sperm DNA fragmentation as part of a genomic screening mechanism developed to genetically inactivate sperm with a defective genomic makeup.


Key words: Sperm • Aneuploidy • DNA Fragmentation • Sperm Chromatin Dispersion Test




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D. T. Carrell
The Clinical Implementation of Sperm Chromosome Aneuploidy Testing: Pitfalls and Promises
J Androl, March 1, 2008; 29(2): 124 - 133.
[Abstract] [Full Text] [PDF]


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