Journal of Andrology
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Published-Ahead-of-Print April 1, 2006, DOI:10.2164/jandrol.05185

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Testis-Specific Lactate Dehydrogenase (LDH-C4; Ldh3) in Murine Oocytes and Preimplantation Embryos

Scott Coonrod , Alejandra Vitale , Chongwen Duan , Sarah Bristol-Gould , John Herr , and Erwin Goldberg

LDH-C4 (Ldh3) is a member of the lactate dehydrogenase family of isozymes that catalyze the terminal reaction in the glycolytic pathway. In mammals three genes, ldha,

ldhb, and Ldhc encode the subunits that assemble into catalytically active homo- and heterotetramers. Differential expression of these genes determines the LDH isozyme composition of tissues and as is well known, A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart with the Ldh2 isozyme the most abundant form in oocytes. The C peptide can be detected first in pachytene spermatocytes and constitutes the primary LDH of spermatozoa. Originally the Ldhc gene (Ldh3 in terminology applied to murine cells) was considered to be testis-specific on the basis of immunochemical, enzymatic and molecular analyses. Here we report the detection of this isozyme in the murine oocyte and early embryo. Our results indicate that Ldh3 mRNA is transcribed in oocytes, and cannot be detected in fertilized eggs. Ldh3 protein, however, persists to the blastocyst stage of embryonic development localizing mainly to the cortex region of oocytes, eggs, zygotes and embryonic blastomeres.




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F. Odet, C. Duan, W. D Willis, E. H Goulding, A. Kung, E. M Eddy, and E. Goldberg
Expression of the Gene for Mouse Lactate Dehydrogenase C (Ldhc) Is Required for Male Fertility
Biol Reprod, July 1, 2008; 79(1): 26 - 34.
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