Journal of Andrology
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Published-Ahead-of-Print April 5, 2006, DOI:10.2164/jandrol.05143

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The Length of the Spermatogenic Cycle is Conserved in Porcine and Ovine Testis Xenografts

Wenxian Zeng , Gleide F. Avelar , Rahul Rathi , Luiz R. Franca , and Ina Dobrinski

Xenografting of immature mammalian testis tissue into mice can accelerate sperm production. To determine whether this shortened time to sperm production is due to reduced length of the spermatogenic cycle, we applied bromodeoxyuridine (BrdU) incorporation to analyze the spermatogenic cycle in porcine and ovine testis xenografts. Small testis fragments from newborn pigs and sheep were ectopically grafted into mice. Once complete spermatogenesis was present in grafted tissue, mice were injected with BrdU and grafts were recovered at different time points thereafter. In porcine grafts, the most advanced germ cells labeled 1 h, 9 d, 12.3 d, and 18 d after BrdU injection were stage one pre-leptotene/leptotene primary spermatocytes, stage one pachytene primary spermatocytes, stage five newly-formed round spermatids, and late stage two elongating spermatids, respectively. In ovine grafts, the most advanced labeled germ cells at 1 h, 11 d and 22 d post BrdU injection were stage two pre-leptotene/leptotene primary spermatocytes, late stage one pachytene primary spermatocytes and stage two elongating spermatids, respectively. These results indicate that each spermatogenic cycle in porcine and ovine xenografts lasts approximately 9 and 11 days, respectively, which is similar to that in situ. Therefore, the length of the spermatogenic cycle is conserved in porcine and ovine testis xenografts. This is consistent with earlier reports showing that the cycle length is inherent to the germ cell genotype. The shortened time to sperm production in xenografts therefore appears due to accelerated maturation of the testicular somatic compartments. Our results suggest that testis xenografts provide a useful model to study the timing of testicular maturation and spermatogenesis in different mammalian species.

Key Words: pig; sheep; graft; spermatogenesis; seminiferous epithelium cycle




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