Journal of Andrology Free Medline Services
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Published-Ahead-of-Print December 8, 2005, DOI:10.2164/jandrol.05107

This Article
Right arrow Author Manuscript (PDF)
Right arrow All Versions of this Article:
27/3/459    most recent
Author Manuscript (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baumber, J.
Right arrow Articles by Meyers, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baumber, J.
Right arrow Articles by Meyers, S. A.

Hyperactivated Motility in Rhesus Macaque (Macaca mulatta) Spermatozoa

Julie Baumber and Stewart A. Meyers *

* To whom correspondence should be addressed. E-mail: smeyers{at}ucdavis.edu .

Macaque spermatozoa can be capacitated according to a defined protocol and exhibit hyperactivated motility similar to that described in other species. The aim of this study was to create a method for defining hyperactivation that could be routinely used in the laboratory alongside our existing sperm motility analysis protocol. Percoll separated macaque spermatozoa were incubated for 2 hours (37°C; 5 % CO2 in air) at a concentration of 20 x 106/mL in bicarbonate (36 mM)-buffered BWW containing 30 mg/ml BSA, followed by an additional 30 minutes with (Capacitated) or without (Incubated) caffeine (1 mM) and dibutyryladenosine 3',5'-cyclic monophosphate (dbcAMP;1.2 mM). One hundred and fifty progressive and hyperactivated tracks were selected from each of three monkeys. Thresholds for hyperactivation were based on the 10th (ALH) and 90th percentile (LIN) of the hyperactivated kinematic data set and were linearity (LIN) ≤ 69 % and amplitude of lateral head displacement (ALH) ≥ 7.5 µM; a threshold of ≥ 130 µM/sec was also included for curvilinear velocity (VCL). These thresholds were 91 % effective at identifying hyperactivated tracks. Capacitation of macaque spermatozoa, by the addition of caffeine and dbcAMP, resulted in a significant increase in ALH, VCL and beat cross frequency and a significant decrease in total and progressive motility, straight line velocity, straightness and LIN when compared to incubated spermatozoa; suggesting the expression of hyperactivated motility. Utilizing the above thresholds, hyperactivation was expressed by 5 ± 0.8 % of the incubated sperm population versus 53 ± 3.7 % of the capacitated sperm population (P < 0.0001). Hyperactivation was not observed when dbcAMP and caffeine were added separately and was significantly (P < 0.005) reduced by the addition of H-89. The results of this paper demonstrate that hyperactivation can be reliably estimated for rhesus macaque spermatozoa.




This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
G. R. Hunnicutt, D. E. Koppel, S. Kwitny, and A. E. Cowan
Cyclic 3',5'-AMP Causes ADAM1/ADAM2 to Rapidly Diffuse Within the Plasma Membrane of Guinea Pig Sperm
Biol Reprod, November 1, 2008; 79(5): 999 - 1007.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P.-h. Hung, M. G. Miller, S. A. Meyers, and C. A. VandeVoort
Sperm Mitochondrial Integrity Is Not Required for Hyperactivated Motility, Zona Binding, or Acrosome Reaction in the Rhesus Macaque
Biol Reprod, August 1, 2008; 79(2): 367 - 375.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2005 by The American Society of Andrology.