Journal of Andrology Proceedings of the Fifth International Conference on the Epididymis
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Published-Ahead-of-Print June 10, 2010, DOI:10.2164/jandrol.110.010306
Journal of Andrology, Vol. 31, No. 6, November/December 2010
Copyright © American Society of Andrology
DOI: 10.2164/jandrol.110.010306

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Insulin Affects Tissue Organization and the Kinetics of Epithelial Cell Death in the Rat Ventral Prostate After Castration

DANILO M. DAMAS-SOUZA*, CLÁUDIO A. OLIVEIRA{dagger} AND HERNANDES F. CARVALHO*

From the * Department of Anatomy, Cell Biology, Physiology, and Biophysics, Institute of Biology, State University of Campinas (UNICAMP), Campinas SP, Brazil; and the {dagger} Department of Animal Reproduction, College of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo SP, Brazil.

Correspondence to: Hernandes F Carvalho, Department of Anatomy, Cell Biology, Physiology, and Biophysics–UNICAMP, CP6109, 13083-863 Campinas SP, Brazil (e-mail: hern{at}unicamp.br).



Abstract

Prostate growth and physiology are regulated by steroid hormones and modulated by multiple endocrine factors. We investigated the action of insulin on the tissue organization and kinetics of epithelial cells in the rat ventral prostate (VP) in response to castration up to 120 hours after surgery by using an acute protocol of alloxan-induced diabetes. Diabetes caused a reduction in volume density (Vv%) and volume of the epithelium. The effects of castration on the epithelium were accelerated in the diabetic animals, as determined by changes in Vv% and volume. The smooth muscle cells became atrophic and apparently relaxed in response to castration, in contrast to the spinous aspect observed in nondiabetic castrated rats. Counting of apoptotic nuclei in the epithelium showed the classical apoptosis peak at 72 hours in nondiabetic rats and an advance of the apoptosis peak to 48 hours after castration in diabetic rats. Insulin restored the time of the peak to 72 hours. These results were confirmed after immunostaining for cleaved caspase-3 and suggest a survival and antiapoptotic effect on VP epithelial cells in both the presence and absence of androgen stimulation. This idea is supported by the observation that insulin also reduced the overall rate of apoptosis at all experimental points analyzed before and after castration.

     Key words: Apoptosis, tissue remodeling






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