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Journal of Andrology, Vol. 27, No. 4, July/August 2006
Copyright © American Society of Andrology

Testis-Specific Lactate Dehydrogenase (LDH-C₄; Ldh3) in Murine Oocytes and Preimplantation Embryos

CHONGWEN DUAN

SARAH BRISTOL-GOULD

JOHN HERR

ERWIN GOLDBERG

From the ^* Department of Genetic Medicine, Weill Medical College of Cornell University, New York, New York; Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois; Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois; and Department of Cell Biology, University of Virginia, Charlottesville, Virginia.

Correspondence to: Dr Erwin Goldberg, Department of Biochemistry, Molecular Biology and Cell Biology, 2205 Tech Dr, Northwestern University, Evanston, IL 60208 (e-mail: erv{at}northwestern.edu).

LDH-C₄ (Ldh3) is a member of the lactate dehydrogenase family of isozymes that catalyze the terminal reaction in the glycolytic pathway. In mammals, 3 genes, ldha, ldhb, and Ldhc, encode the subunits that assemble into catalytically active homo- and heterotetramers. Differential expression of these genes determines the lactate dehydrogenase (LDH) isozyme composition of tissues, and, as is well known, A subunits predominate in skeletal muscle and B subunits are abundantly produced in brain and heart, with the Ldh2 isozyme the most abundant form in oocytes. The C peptide can be detected first in pachytene spermatocytes and constitutes the primary LDH of spermatozoa. Originally the Ldhc gene (Ldh3 in terminology applied to murine cells) was considered to be testis specific on the basis of immunochemical, enzymatic, and molecular analyses. Here we report the detection of this isozyme in the murine oocyte and early embryo. Our results indicate that Ldh3 mRNA is transcribed in oocytes and cannot be detected in fertilized eggs. Ldh3 protein, however, persists to the blastocyst stage of embryonic development localizing mainly to the cortex region of oocytes, eggs, zygotes, and embryonic blastomeres.

     Key words: Ovary, gene expression, glycolysis, enzyme

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