Figure 5. Immunohistochemical detection of SOX9 (pink), anti-Müllerian hormone (green), and Laminin 1 (Lama1; white) in newborn Wnt4–/–;Foxl2–/– double knockout ovary showing testis tubules and extensive Sox9 expression (A, B) that are absent in newborn Wnt4–/–;Foxl2+/– knockout ovary (C) and present in a control testis (D). Large tubular structures that are located near the hilum of the dysgenetic ovaries and express SOX9 but not AMH (A–C) correspond to the rete and directly communicate with malelike internal genitalia. Nuclei are counterstained with 4',6-diamidino-2-phenylindole (blue).