| |
Etoposide
|
Doxorubicin
|
Okadaic acid
|
| Cell Line |
Concentration, µg/mL |
Colonies, % Control |
Concentration, µg/mL |
Colonies, % Control |
Concentration, nM |
Colonies, % Control |
|
| PNT2C2 |
5 |
0.41 ± 0.12 (4) |
0.25 |
2.29 ± 0.20 (6) |
10 |
16.80 ± 6.30 (3) |
| |
10 |
0.05 ± 0.02 (3) |
0.50 |
0.08 ± 0.03 (3) |
50 |
0.33 ± 0.07 (5) |
| |
25 |
<0.03 |
1.00 |
<0.03 |
100 |
0.13 ± 0.08 (2) |
| P4E6 |
ND |
ND |
0.20 |
<0.60 |
ND |
ND |
| 22Rv1 |
50 |
<0.09 |
0.20 |
8.18 ± 1.69 (6) |
20 |
<0.06 |
| |
|
|
0.50 |
<0.03 |
|
|
| LNCaP |
200 |
0.22 ± 0.01 (3) |
0.20 |
0.75 ± 0.18 (3) |
20 |
<0.06 |
| |
|
|
0.50 |
<0.09 |
|
|
| PC-3 |
200 |
3.90 ± 2.06 (4) |
0.25 |
<2.50 |
10 |
7.00 ± 3.00 (2) |
| |
|
|
|
20 |
0.39 ± 0.31 (3) |
|
| |
|
|
|
50 |
<0.08 |
|
|
| Abbreviation: ND, not determined. |
| aCells were treated with the indicated concentrations of chemical agent (or
vehicle for controls). After 72 hours, cells were trypsinized, washed, and
then replated. The number of colonies formed was counted after 1-2 weeks; data
are means ± SEM (number of experiments is given in parentheses) and are
expressed as a percentage of control cells. In cases where no colonies were
formed, the detection limit of the assay is recorded. |
|
