Figure 1. Reverse transcriptase polymerase chain reaction (RT-PCR) screening for hLdhc expression in 4 melanoma cell lines (see text for description). Upper panel: RT-PCR for hLdhc; bottom panel: β-actin control. A375P and A375M melanoma cell lines are isogenic. C8168 and C81-61 are isogenic. Multiple transcripts of hLdhc expression were detected in A375M and C81-61 cells. The top (slowest-migrating) band on the gel was the full-length cDNA based on sequencing data. The other bands are cancer-specific splicing variants of the gene.