| Primary Antibodies (Catalog No.) |
Source |
Dilution |
Secondary Antibodies (Manufacturer) |
Testis Target Cells |
Species Specificitya |
|
Anti- -smooth muscle actin (A-2547) |
Sigma |
1:3000-6000 |
Rabbit anti-mouse (Chemicon) |
Peritubular myoid & smooth muscle cells |
Mouse Bull |
| Anti-vimentin (Clone v9) |
Dako Australia, Kingsgrove, Australia |
1:3000-5000 |
Rabbit anti-mouse (Chemicon) |
Sertoli cells |
Bull |
| WT1 (C-19, sc-192) |
Santa Cruz Biotech, CA |
1:500-1000 |
Rabbit anti-sheep (Chemicon) |
Sertoli cells |
Mouse Bull |
| MIS (sc-6886) |
Santa Cruz Biotech |
1:100-200 |
Rabbit anti-sheep (Chemicon) |
Sertoli cells |
Mouse Bull |
| NONO |
Dr Kuriharab |
1:200 |
Rabbit anti-mouse (Chemicon) |
Sertoli cells |
Mouse |
| GCNA1 |
Dr Endersb |
1:50-100 |
Goat anti-rat (Dako) |
Germ cells |
Mouse |
| DDX4/MVH (ab13840) |
Abcam, Cambridge, MA |
1:2000-4000 |
Goat anti-rabbit (Chemicon) |
Germ cells |
Mouse Bull |
| PGP 9.5c (AB5925) |
Chemicon, Temecula, CA |
1:1000-13 000 |
Goat anti-rabbit (Chemicon) |
Spermatogonia |
Mouse Bull |
| Lectin (DBA) (L6533 or L9142) |
Sigma |
1:100-200 |
Not applicabled |
Spermatogonia |
Bull |
|
| aSpecies specificity is based on our experimental results. |
| bReferences: Enders and May,
1994; Kuwahara et al,
2006. |
| cPGP9.5: Protein Gene Product 9.5. |
| dDolichos biflorus agglutinin (DBA) is not an antibody. It is
conjugated with biotin (L6533) or FITC (L9142), so secondary antibody is not
used. |
