Figure 6. MEHP exposure and COX-2 induction. (A) Whole-cell lysates (15 µg), (B) mitochondrial (15 µg), or (C) cytosolic (15 µg) proteins were isolated from cells treated with 0.05% DMSO (vehicle) or 100 or 200 µM MEHP (24 hours). Sequential Western blot analyses; signals are normalized to (A, C) β-actin or (B) VDAC, respectively. For each experiment, the average vehicle values were (A) 0.862, (B) 0.868, and (C) 0.638. Data are expressed as arbitrary units relative to vehicle, set as 1. Error bars indicate SEM, * P < .01 indicates significance differences from vehicle by ANOVA with Dunnett multiple comparison. A representative Western blot is shown beneath. (A) n = 3, (B, C) n = 4 each.