| Treatment |
+ESP/-AcR |
+ESP/+AcR |
-ESP/-AcR |
-ESP/+AcR |
Total |
% +AcR |
% +ESP |
% AcR Also +ESP |
|
| Noncapacitated |
537 (91.8) |
31 (5.3) |
15 (2.6) |
2 (0.3) |
585 |
5.6 |
97.1 |
93 |
| Capacitated |
464 (85.1) |
68 (12.5) |
8 (1.5) |
5 (1.0) |
545 |
13.4 |
97.6 |
93 |
| EtOH |
456 (83.4) |
70 (12.8) |
12 (2.2) |
9 (1.6) |
547 |
14.4 |
96.2 |
89 |
| Progesterone |
438 (79.5) |
87 (15.8) |
15 (2.7) |
11 (2.0) |
551 |
17.8 |
95.3 |
89 |
|
a Presence of ESP in acrosome-reacted sperm. Fresh swim-up sperm from 5
individuals were dually stained with PSA-lectin and  —rec-ESP
antibody. The acrosome reaction was assessed by loss of PSA lectin staining
from the principal segment of the acrosome. Samples were scored as -AcR,
indicating full PSA-positive acrosomal cap (principal segment), or +AcR,
indicating diminution or absence of a PSA-positive principal segment. Samples
consisted of noncapacitated swim-up sperm, capacitated sperm treated with
ethanol vehicle alone, and capacitated sperm treated with 5 µg/mL
progesterone. ESP was retained in 89% of acrosome-reacted sperm, identified by
a decrease or absence of PSA staining. The numbers in the parentheses indicate
percentage of total sperm observed in the category. |
|
