Figure 4. Luciferase activity of the deletion constructs of the 5'-flanking region of thymosin β4 in LNCaP cells. LNCaP cells were transfected with 2650pGL3, 695pGL3, 403pGL3, 193pGL3, 83pGL3, or 46pGL3 and pRL-CMV as described in "Materials and Methods." After 72 hours, the cell lysates were prepared, and luciferase activities were measured. The luciferase activity in cells incubated in the medium containing 5% FCS was taken as 100%. Data represented are the mean ± SD from at least 2 independent experiments, each performed in triplicate. * indicates P < .05; **, P < .01; ***, P < .001.