| Parameter |
PPCA +/+ mice Mean ± SD (# of observations) |
PPCA –/– mice Mean ± SD (# of observations) |
Change |
P |
|
Power|| |
|
| Cauda epididymis |
|
|
|
|
|
|
| Sperm counts, millions/mL |
23.3 ± 4.8 (147) |
7.1 ± 3.2 (116) |
–70% |
.0000 |
|
1.0000 |
| For all cases in data set |
|
|
|
|
|
|
| Raw values |
(102) |
(58) |
|
|
|
|
| VAP |
61.2 ± 1.5 |
52.5 ± 19.1 |
–14% |
.0010 |
|
.9454 |
| VSL |
5.1 ± 9.3 |
41.6 ± 16.3 |
–17% |
.0002 |
|
.9762 |
| VCL |
87.8 ± 15.6 |
86.5 ± 29.4 |
–1% |
.7467 |
NS |
.0653 |
| ALH |
2.5 ± .6 |
3.2 ± 2.0 |
28% |
.0046 |
|
.8711 |
| BCF |
.9 ± 1.7 |
2.2 ± 4.7 |
144% |
.0303 |
|
.6679 |
| Ratios |
|
|
|
|
|
|
| STR |
81.0 ± 9.2 |
77.8 ± 7.1 |
–4% |
.6283 |
NS |
.0534 |
| LIN |
58.8 ± 7.2 |
49.8 ± 9.9 |
–15% |
.2724 |
NS |
.1545 |
| Elong(ation)¶ |
46.2 ± 4.8 |
5.1 ± 7.9 |
8% |
.6356 |
NS |
.0544 |
| Percentages |
|
|
|
|
|
|
| Motile |
85.3 ± 7.3 |
45.0 ± 24.5 |
–47% |
.0000 |
|
.9995 |
| Prog(ressive) |
33.6 ± 12.3 |
13.1 ± 12.3 |
–61% |
.0052 |
|
.7836 |
| Rapid |
5.2 ± 13.6 |
2.3 ± 16.9 |
–60% |
.0003 |
|
.9612 |
| Medium |
35.2 ± 12.9 |
24.6 ± 15.1 |
–30% |
.1667 |
NS# |
.2192 |
| Slow |
2.7 ± 4.1 |
5.4 ± 6.9 |
100% |
.3844 |
NS# |
.0799 |
| Static |
12.0 ± 7.0 |
49.7 ± 25.8 |
314% |
.0000 |
|
.9989 |
For only those cases in PPCA –/– mice with percent static  30% |
|
|
|
|
| Raw values |
same as above |
(42) |
|
|
|
|
| VAP |
|
51.8 ± 2.8 |
–15% |
.0025 |
|
.9294 |
| VSL |
|
41.0 ± 17.8 |
–18% |
.0007 |
|
.9661 |
| VCL |
|
88.5 ± 33.2 |
1% |
.8923 |
NS |
.0534 |
| ALH |
|
3.4 ± 2.3 |
36% |
.0017 |
|
.9421 |
| BCF |
|
2.7 ± 5.4 |
200% |
.0115 |
|
.8423 |
| Ratios |
|
|
|
|
|
|
| STR |
|
77.0 ± 7.9 |
–5% |
.6824 |
NS |
.0589 |
| LIN |
|
47.7 ± 1.0 |
–19% |
.2251 |
NS |
.1778 |
| Elong(ation)¶ |
|
51.1 ± 8.4 |
11% |
.5858 |
NS |
.0576 |
| Percentages |
|
|
|
|
|
|
| Motile |
|
33.5 ± 16.8 |
–61% |
.0000 |
|
1.0000 |
| Prog(ressive) |
|
9.0 ± 1.1 |
–73% |
.0025 |
|
.8643 |
| Rapid |
|
14.1 ± 13.2 |
–72% |
.0001 |
|
.9894 |
| Medium |
|
19.4 ± 13.3 |
–45% |
.0597 |
NS# |
.3760 |
| Slow |
|
4.7 ± 5.9 |
74% |
.5582 |
NS# |
.0500 |
| Static |
|
61.8 ± 18.9 |
415% |
.0000 |
|
1.0000 |
|
| *ALH, amplitude of lateral head displacement (µm); BCF, beat-cross
frequency (Hz); LIN, linearity (ratio of VSL:VCL); NS, not significant; PPCA,
protective protein cathepsin A; STR, straightness (ratio of VSL:VAP); VAP,
smooth-path velocity (µm/sec); VCL, track velocity (µm/sec); and VSL,
straight-line velocity (µm/sec) |
 Total number of observations (measurements) made on a pool of 4 PPCA
+/+ and 3 PPCA –/– mice |
| PPCA –/– mice compared with PPCA +/+ mice |
| P < .05 considered significantly different; Fisher's exact
test was used to compare differences between means for ratios and percentages |
| ||This is the power associated with rejecting the null hypothesis that the
2 means are equal. The Z-test for comparing 2 proportions was used in power
calculations for variables listed under ratios and percentages |
| ¶Head shape (ratio of minor to major axes of sperm head) |
| #The number of observations was too low to establish any significance for
these differences |
