Figure 9. Immunolocalization of HOXB2 homeodomain–containing epididymis-specific sperm protein (HOXBES2) in ligated and unligated epididymis. Localization of HOXBES2 (arrow) was seen in the principal epithelial cells of both ligated and unligated rat epididymis. The figure depicts HOXBES2 localization in the epididymis of a rat sacrificed on postnatal day 15. The fluid volume and sperm count were either reduced or absent in the lumen of the ligated epididymis. P, principal cells; L, lumen; IT, intertubular connective tissue space; S, spermatozoa. Bar = 25 µm. Color figure available online at www.andrologyjournal.org.