Figure 14. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis of the Hoxbes2 gene in rat epididymis. An expected 581-bp RT-PCR product of the Hoxbes2 gene was obtained from 60-day-old adult rat epididymal RNA using Hoxbes2-specific nested primers, forward 5'-CGGCACGAGGACTGCCGG-3' and reverse 5'-GGGTTCTCTCGACAGCCCC-3', which encompass the 581-bp fragment that includes the conserved region showing homology to Hoxb2. No band was seen in the lane of 15-day-old epididymis and the negative control (in which reverse transcriptase or the cDNA template was omitted). A 580-bp ß-actin amplified transcript served as the internal control.