Figure 1. Immunohistochemical localization of HOXB2 homeodomain–containing epididymis-specific sperm protein (HOXBES2) in the castrated rat epididymis. Sections of the epididymal segments from various groups of rats were immunolocalized with a HOXB2 antibody and nonimmune goat serum or secondary antibody alone (negative control) as described under "Materials and Methods" and counterstained with hematoxylin. The data depicted represents staining for the HOXBES2 protein in epididymis of day 7 postcastration rats with DHT supplementation (day 14). The maximum expression of HOXBES2 in the distal segments of epididymis is also shown. P, principal cells; L, lumen; IT, intertubular connective tissue space; S, spermatozoa. Arrows indicate HOXBES2 localization in the principal cells. Bar = 25 µm. Color figure available online at www.andrologyjournal.org.