Figure 5. Adjudin-mediated junction restructuring leads to changes in Rab4A-catenin interactions in the testis. (A) Autoradiograms corresponding to different immunoblots showing an increase in Rab4A–-catenin and Rab4A–ß-catenin coimmunoprecipitation following Adjudin administration. Immunoprecipitation was performed with anti-Rab4A antibody, whereas immunoblotting was performed using antibodies listed to the right of each immunoblot. As a control, anti-Rab4A antibody was used for immunoprecipitation, followed by immunoblotting with the same antibody (A, top panel), and these results were similar to those shown in Figure 4A. IgG served as an indicator of equal protein processing and loading onto gels (A, bottom panel). (B) Densitometric scanning results from at least 3 independent experiments. Each -/ß-catenin data point was first normalized against its corresponding Rab4A data point, followed by a second round of normalizations against testis at 0 hours (control). The control was arbitrarily set at 1. ^*Significantly different from testis at 0 hours by analysis of variance, P < .05; ^**Significantly different from testis at 0 hours, P < .01; ns, not significantly different from testis at 0 hours; IP, immunoprecipitation.