Figure 3. Western blot analysis of protein tyrosine phosphorylation in boar sperm. The upper panels show tyrosine phosphorylation of total proteins from sperm incubated for 3 hours at 39°C (left) or cooled (right) in noncapacitating media (NCM), capacitating media (CM) with and without the inclusion of 10% or 20% (v/v) seminal plasma. The position of p32, a protein shown to be phosphorylated during capacitation, is noted. In the lower panels, the blot was stripped and reprobed for beta-tubulin (ß-tub) as a loading control.