Figure 6. Effect of resveratrol on apoptosis index, caspase activation, and bax/bcl-2 expression in prostate cancer cell lines. LNCaP, PC-3, and PZ-HPV-7 cell cultures were treated with 0–150 µM of resveratrol for 36 hours. Representative histograms of flow cytometry experiments showing cell distribution upon DNA content in absence or presence of resveratrol (A). The region corresponding to cells with sub-G0/G1 DNA content (considered as apoptotic cells) is indicated as R1. Percentage of apoptotic cells versus resveratrol concentrations in the 3 cell lines (B). Pro- and active forms of caspase-9, -8, and -3 were determined by Western blot (C). Protein expression of bax and bcl-2 were assayed by Western blot (D). ß-actin was used as control. Experiments were carried out at least 3 times with equivalent results. Data were expressed as means ± SEM of 3 different experiments. Photographs shown correspond to representative experiments.