Figure 4. Immunolocalization of ROS-GC1 in the bovine testes. (A) Immunohistochemistry was performed using specific antibodies against ROS-GC1 diluted 1:100 on paraffin section of bovine testes. The ROS-GC1 was localized in spermatogenic cells. The positive staining was observed in spermatogonia (Sg), primary spermatocytes (Sp), and spermatids (Sd), as indicated by the arrows. Bar = 100 µm. (B) When the section were subjected to analysis with anti-S100B antibodies, the majority of positively stained spermatocytes and spermatids were observed. (C) Incubation of paraffin section of bovine testes with antibodies against GCAP1 resulted in staining of only a small population of spermatogonia. (D) Control. The procedure was exactly the same as for detection of ROS-GC1, except that nonimmune serum was used instead of ROS-GC1 antibodies. Lack of staining is characteristic for all cells of spermatogenic cycle.