Figure 6. Tissue sections probed with rabbit anti-rat transferrin antibody followed by FITC-labeled anti-rabbit antibody. (A) Wall of granuloma from day 7 vasectomized rat (200x) showing clear transferrin immunostaining throughout the granuloma, (B) Vas deferens epithelium from day 7 vasectomized rat (200x) showing immunostaining for transferrin in the epithelium and smooth muscle wall, (C) Vas deferens from a sham operated rat (200x) showing staining only within the epithelium, (D) Caput epididymis from a sham operated rat (200x) showing cytoplasmic staining of many epithelial cells and intense staining in the interstitial space, (E) Testis from a sham operated rat (200x) showing cytoplasmic staining in Sertoli cells and intense staining in the interstitial space, (F) Testis from a sham operated rat probed with secondary antibody only (200x) showing a complete lack of nonspecific staining.