Figure 4. Epididymal spermatozoa real-time polymerase chain reaction genotyping for the deleted IB kinaseb (IKKß) allele and Y chromosome. All 4 genotypes have approximately 50% Y sperm (right panel) as a control. The percentage of sperm with the allele is at background levels for Ikkß^F/F and Cre-Ikkß^F/F in late-generation mice, while approximately 45% for Ikkß^F/ and Cre-Ikkß^F/ mice, indicating a lack of floxed recombination. Experiments were performed in triplicate with a housekeeping gene as an internal control. (n > 3 for all genotypes).