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Motility data after 2.5 hours in the respective buffers at room temperature or 37°C*

Sample	TM (%)	PM (%)	VAP	VSL	VCL	ALH	BCF	STR	LIN

RT Control ${dagger}$	88 ± 2.0	71 ± 2.6^a	149 ± 9.6	137 ± 9.3	178 ± 6.7	4.1 ± 0.3^a	28 ± 1.8^a	89 ± 0.9^a	75 ± 3.2^a
Incubated ${ddagger}$	81 ± 4.7	63 ± 6.6^a,b	154 ± 13.7	145 ± 13.0	172 ± 12.9	3.2 ± 0.2^b	28 ± 1.2^a	91 ± 1.5^a	79 ± 4.0^a
Capacitated ${ddagger}$	77 ± 5.4	53 ± 6.7^b	135 ± 15.3	118 ± 14.5	186 ± 14.6	5.9 ± 0.3^c	34 ± 1.04^b	85 ± 1.1^b	61 ± 3.2^b

*Mean values (± SEM) with different superscripts within columns (^a,b,c) are statistically different (P < .05).
${dagger}$ Conditions for the room temperature (RT) control were as follows: Hepes-BWW plus 1 mg/mL BSA.
${ddagger}$ Conditions for the 37°C samples were as follows: incubated group, Bicarb-BWW plus 30 mg/mL BSA; and capacitated group, Bicarb-BWW plus 30 mg/mL BSA, final 30 minutes with 1.2 mmol/L dbcAMP and 1 mmol/L caffeine.