Figure 6. Western blot of tissue extracts probed with antibody to Ldh3. One hundred germinal vesicle (GV) oocytes were harvested from 5- to 6-week-old mouse ovaries. One hundred metaphase II (MII) oocytes were collected from superovulating females with injections of pregnant mare's serum gonadotropin (5 U per mouse) and human chorionic gonadotropin (5 U per mouse). Ldh3 was purified from mouse testis, and 100 ng was loaded on the gel. Heart was homogenized in extraction buffer containing 1% Triton X-100, and 20 µg protein was loaded. Lane 1, GV oocytes; lane 2, MII oocytes; lane 3, Ldh3; lane 4, heart extract. Specific signals were obtained in lanes 1–3 only.