Figure 5. Prostaglandin D2 synthase (Ptgds) gene expression in segments 1
and 2 vs segments 9 and 10 in the mouse epididymis. Duplicate RNA samples (+)
extracted from segments 1 and 2 and segments 9 and 10 were used in
semiquantitative reverse transcriptase–polymerase chain reaction
(RT-PCR) for Ptgds and illustrate that the gene is more highly expressed in
the distal segments than in the proximal segments. This is consistent with the
results of the microarray analysis of Ptgds expression in all 10 segments of
the mouse epididymis (Figure
4B). No RT lanes (-) were negative for PCR product, nor were
no-template lanes (not shown).
