| Regression Equations That Include, as Independent Variables, Intensity of
Spots* on AGF Protein Maps |
R2 and P Value |
|
1) PD = –7.0–0.0005 x (spermadhesin) + 0.00015
x (osteopontin) + 0.0042 x (BSP 30 kDa)–3.59 x
10–7 x (BSP 30 kDa)2 |
R2 = .56, P < .0001 |
| 2) PD = –8.15–0.0005 x (spermadhesin) + 0.0026 x
(phospholipase A2) + 0.0048 x (BSP 30 kDa)–4.14 x
10–7 x (BSP 30 kDa)2 |
R2 = .53, P < .0001 |
|
| *Spot intensity given as ppm of the total integrated optical density, as
calculated by PDQest software |
| PD indicates percentage point deviation from the average nonreturn
rate of bulls; (spermadhesin): average intensity of two 14-kDa spermadhesins
(spots 2201 and 2202); (osteopontin): intensity of a 55-kDa osteopontin (spot
9802); (BSP 30 kDa): average intensity of five BSP 30 kDa isoforms (spots
7303–8403); (phospholipase A2): average intensity of 2
isoforms of 58-kDa phospholipase A2 (spots 3801 and 4801), as shown
in Figure 2 |
|
