| |
|
Relative Levels of mBBr-Fluorescence of Sperm Heads
|
| Spermatozoa |
|
Untreated |
Treated
|
EF |
GSH |
CSH |
GSSG |
CSSC |
|
| Caput |
— |
10 |
10 |
10 |
1 |
1 |
| Caput |
+ |
1 |
3 |
5 |
1 |
1 |
| Cauda |
– |
1 |
10 |
10 |
1 |
1 |
| Cauda |
+ |
1 |
1 |
1 |
1 |
1 |
|
| *GSH indicates glutathione; CSH, cysteine; GSSG, glutathione disulfide; CSSC,
cysteine disulfide; and mBBr, monobromobimane. |
| Washed (–epididymal fluid [EF]) or unwashed (+EF) caput and
cauda sperm suspensions were kept at 4°C for 3 days without or with 1 mM
GSH, CSH, GSSG, and CSSC; spermatozoa were then washed and labeled with mBBr.
The fluorescence intensity of the sperm heads, evaluated by microscopy, was
graded from 1 (very low intensity) to 10 (very high intensity). |
|
