Figure 5. Effect of spinal cord transection (SCX) and exogenous hormones on sperm protein phosphorylation. (A) A representative Western blot showing lower intensities of phosphorylated protein bands in spermatozoa of SCX rats. (B) Quantitative comparison of intensity of the major phosphorylated sperm protein between sham control and SCX rats. Results are expressed as mean ± SEM of 4 rats. a, P < .05 vs sham control. (C, D) Representative Western blots of sham control and SCX rats, respectively, receiving various hormone treatments. Each blot contained samples randomly selected from each experimental group. (E) Intensity of the major phosphorylated protein band was scanned and normalized against that of the untreated control in each blot. Results are expressed as mean ± SEM of 4 blots.