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Table 7. Capacitation status; subpopulations of spermatozoa after staining with merocyanine 540 and Yo-Pro 1 and examined by flow cytometry; boar frozen spermatozoa with freezing media (lactose egg yolk) supplemented (1 and 5 mM) or not with reduced glutathione^*

Freezing Media   Viable Capacitated   Viable Capacitated   Not Dead   Control   8.66 ± 0.99   35.27 ± 2.64 A   56.06 ± 2.99 A   1 mM GSH   9.06 ± 0.53   48.63 ± 2.27 B   42.30 ± 2.00 B   5 mM GSH   8.97 ± 0.71   40.28 ± 2.17 AB   50.74 ± 1.85 AB   ANOVA, P values   .9181   .003   .001   *Numbers within columns with different letters differ (P < .05)   Averages data from 6 freezing batches   GSH indicates glutathione; ANOVA, analysis of variance

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