Journal of Andrology Proceedings of the Fifth International Conference on the Epididymis
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Table 2. Isotopic values (m/z) of peptides obtained from an in-gel, tryptic digest of the fSP8 protein determined by MALDI-TOF mass spectrometry, and theoretical values calculated from the amino acid sequence*
    m/z
  Peptide Sequence, Position, and Modifications   Calculated   Measured
      1 (–)MKRGSAALEVR(E)11 1Met-ox 1 AcetK 1Myr 1GuanK 1A-SAM   1927.04   1927.04
      3 (K)RGSAALEVRELK(M) 14 1AcetK 3Myr 1GuanK 1PLP   2273.41   2273.25
  49 (R)EVMMAARK(G) 56 1AcetK 1Myr 1GuanK 2A-SAM   2027.98   2027.97
  56 (R) KGLDPYNILAPK (A) 67   1328.76   1328.73
  57 (K) GLDPYNILAPK (A) 67   1200.66   1200.63
  68 (K) AAAGTKEDPNLVPSITNKR (I) 86   1982.07   1982.12
  *Met-ox indicates oxidation of M; AcetK, acetylation of K; Myr, myristilation; and Guan, guanidylation





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