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Table 3. Effect of the addition of catalase (CAT) and superoxide dismutase (SOD) in combination in the freezing extender on the postthaw boar sperm motilty, kinematic parameters, and viability and in their ability to develop embryos in vitro

Treatment CAT + SOD, IU/mL       Postthaw Sperm Parameters*   0 (Control)   200+150   400+300   SEM   Probability, P   Motility and kinematic parameters                 TMS, %   57.82a   68.43b   67.64b   3.59   <.01       RPMS, %   15.38a   19.80b   20.05b   2.72   <.01       VAP, µm/s   31.87a   34.81b   35.23b   2.19   <.05       LIN, %   59.06a   55.19b   55.56b   1.33   <.01       ALH, µm   1.42a   1.92b   1.83a,b   0.16   <.05       BCF, Hz   7.76a   8.75b   8.52b   0.23   <.01   Viable sperm with intact acrosome, %   58.89a   70.23b   69.98b   3.45   <.01   In vitro embryo production                 Inseminated oocytes, No.   240   240   240           Cleaved zygotes, No. (%)   74 (30.83)a   120 (50)b   130 (54.17)b   3.3   <.01       Blastocyst formation, No. (%)   42 (17.50)a   76 (31.67)b   76 (31.67)b   2.8   <.01       % cleaved reaching blastocyst   56.76   63.33   58.46   4.5   NS   *TMS, total motile spermatozoa; RPMS, rapid progressive motile spermatozoa (>50 µm/s); VAP, average path velocity; LIN, linearity; ALH, amplitude of lateral head displacement; and BCF, beat cross frequency. Pooled data from 7 replicates. Motility and viability value are the mean of the 2 separate determinations at 30 and 150 minutes postthawing in samples incubated for 150 minutes at 37°C.   Different superscript letters denote significant differences (P < .05).

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