Figure 8. In situ localization of Spergen-3 mRNA in the seminiferous tubules of adult rat testis (A–C) and of developing rat testis (E–H). Frozen sections are hybridized either with a DIG-labeled cRNA probe (A–C, E–H) or with a sense probe (D). In adult testis, expression of Spergen-3 mRNA in the seminiferous tubules of stage I–III (A), stage VII–VIII (B), and stage IX–XI (C) are shown. Hybridization with a sense probe gives no specific signal (D). In developing rat testis, expression of Spergen-3 mRNA in postnatal 2-week (E), 3-week (F), 4-week (G), and 5-week (H) are shown. Bars = 30 µm (A–D), 50 µm (E–H).