Journal of Andrology Proceedings of the Fifth International Conference on the Epididymis
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Table 4. Effect of sperm cleansing (by centrifugation in saline bovine serumalbumin or through discontinuous Percoll gradient separation) of frozen-thawed spermatozoa (single batch) previously incubated for 120 minutes at 38°C with PSP-I/PSP-II heterodimer (1.5 mg/mL) on their capability to in vitro penetrate in vitro matured oocytes*
  Group   Oocytes Penetrated (%)   Oocytes Polispermic (%)   Mean Spermatozoa per Oocyte (No.)
  Control   91.1 ± 2.2 A{dagger}   68.7 ± 1.9 A   2.6 ± 0.2 A
      (n = 402)      
  Exposed to PSP-I/II   30.8 ± 3.6 B   36.4 ± 2.7 B   1.4 ± 0.1 B
      (n = 395)      
  Exposed to PSP-I/II and washed by centrifugation   12.8 ± 1.8 C   25.1 ± 2.3 C   1.6 ± 0.1 B
      (n = 390)      
  Exposed to PSP-I/II and cleansed by Percoll gradient centrifugation   53.1 ± 4.2 D   38.5 ± 2.9 B   1.4 ± 0.2 B
      (n = 405)      
  *Data are expressed as mean ± SEM
  {dagger}Different letters in the same column indicate significantly different values (P < .05)





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