| Operation |
Global Parameters |
GAPDH |
ANG |
AT-I |
|
| Activation of the polymerase |
10 min, 95°C |
|
|
|
| Denaturation |
0 s, 95°C |
|
|
|
| Annealing |
|
58°C |
62°C |
58°C |
| Elongation |
|
72°C, 20 s |
72°C, 20 s |
72°C, 20 s |
| Number of PCR cycles |
|
40 |
50 |
40 |
| Melting |
75°C to 95°C |
|
|
|
| Cooling |
95°C to 40°C |
|
|
|
| Melting peak temperature of amplified fragments |
|
91.5°C |
90°C |
84°C |
|
| *GAPDH indicates glyceraldehyde phosphate dehydrogenase; ANG, angiotensinogen;
AT-I, angiotensin II type I receptor; and PCR, polymerase chain reaction |
|
