| |
|
|
GenBank Accession Number |
|
|
CCR5 Transcript Copies Shared per 106 Heterogeneous Spermatozoa
Population |
Number of Positive Individuals
|
| Primer |
Sequence (5'-3' direction) |
Position |
Exons |
Product Size |
Spermatozoa |
PBMCs |
|
| E1 |
5'-ATATCTGGAGTGAAGGATCCT-3' |
14-34 |
AF031237 |
1,2 |
140 bp |
0 |
0 |
13 |
| E2a |
5'-GGTCTCTAGATAAGAGATCGA-3' |
634-654 |
|
|
|
|
|
|
| E1 |
5'-ATATCTGGAGTGAAGGATCCT-3' |
14-34 |
AF031237 |
1,3 |
313 and 78 bp |
0 |
0 |
13 |
| E3a |
5'-GGAACGGATGTCTCAGCTC-3' |
809-827 |
|
|
|
|
|
|
| E2b |
5'-TTTAACTCCACCCTCCTTCAA-3' |
722-742 |
AF031237 |
|
106 bp |
0 |
0 |
13 |
| E3b |
5'-GGAACGGATGTCTCAGCTC-3' |
809-827 |
|
|
|
|
|
|
| E3b |
5'-GAGCTGAGACATCCGTTCC-3' |
809-827 |
AF031237 |
2,3 |
139 bp |
20 320 ± 11 990 |
13 |
13 |
| E4 |
5'-GCTGCGATTTGCTTCACATTG-3' |
2830-2850 |
|
3,4 |
|
|
|
|
| E5 |
5'-GGCAAAGACAGAAGCCTCAC-3' |
4452-4471 |
AF031237 |
4 |
101 bp |
18 920 ± 9780 |
13 |
13 |
| E6 |
5'-CTAGCATTGACCTTCCTCCC-3' |
4533-4552 |
|
|
|
|
|
|
| MYP |
5'-CCTTGCTGGGCTGGGGGTCTCAC-3' |
226-248 |
NM 000250 |
1,3 |
394 bp |
0 |
0 |
13 |
| |
5'-GGGCGGGCGTCAGCACATCAG-3' |
559-619 |
|
|
|
|
|
|
| GPDH |
5'-GTCTGTCGTGGACCTGACCT-3' |
1002-1021 |
BC036373 |
9,10 |
143 bp |
45 678 ± 5796 |
13 |
13* |
| |
5'-CGGGAAGTCCGTAGAGACG-3' |
1125-1144 |
|
|
|
|
|
|
|
| *Low level of GPDH isoenzyme transcript in PBMCs |
