| Purification Step |
Protein |
Specific Activity (U/mg protein) |
|
| Caput sperm extract |
61.3 mg |
10.73 |
| DEAE-cellulose flow through |
21.7 mg |
15.6 |
| Mono S flow through of DEAE-cellulose flow through |
820 µg |
... |
| Mono S gradient of DEAE-cellulose flow through (0.125-0.165 M KCl) |
104 µg |
64.08 |
|
| *Table 2 summarizes the purification of GSK-3 from 30 mL of caput sperm
extracts as outlined in "Materials and Methods." One unit (U) of
GSK-3 activity is defined as the millmoles of P32 incorporated onto
phospho-CREB/min |
 Abbreviations are explained in the second footnote to
Table 1 |
