Figure 1. The effect that LY294002 (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one) and wortmannin have on the capacitation of human spermatozoa. Spermatozoa resuspended in Biggers-Whitten-Whittingham (BWW) medium were incubated with (A) LY294002 or (B) wortmannin at the indicated concentrations for 30 minutes and then without (white bars) or with fetal cord serum ultrafiltrate (FCSu) (black bars) for 3.5 hours. Sperm capacitation was evaluated as described in "Materials and Methods." Results are mean ± SEM of 5 determinations performed on sperm samples from different donors. ^* indicates that a value is different from that of control spermatozoa (BWW alone), and # indicates that a value is different from that of FCSu-treated spermatozoa.