| |
Concentration of Butylated Hydroxytoluene (mM)
|
| Spermatozoa |
0 |
0.2 |
0.4 |
0.8 |
1.6 |
|
| Live (A-/PI-) |
47.24 ± 2.63 B |
59.19 ± 1.86 A |
57.47 ± 2.10 A |
60.03 ± 1.71 A |
54.85 ± 2.09 AB |
| Early apoptotic (A+/PI-) |
2.68 ± 0.27 |
3.86 ± 0.28 |
3.13 ± 0.31 |
3.14 ± 0.24 |
3.09 ± 0.24 |
| Late apoptotic (A+/PI+) |
21.01 ± 1.72 |
18.98 ± 1.50 |
17.61 ± 1.36 |
17.88 ± 1.51 |
19.00 ± 1.26 |
| Dead (A-/PI+) |
29.06 ± 2.67 A |
15.96 ± 1.47 B |
21.12 ± 2.09 B |
18.96 ± 1.72 B |
23.04 ± 2.01 AB |
|
 Values in the same row with different letters
(A, B) differ (P < .05; n = 6 replicates). |
| *A indicates Annexin V (a component of Annexin V-FITC Apoptosis Detection Kit;
Sigma-Aldrich Co, St Louis, Mo). The Kit includes PI. PI indicates propidium
iodide. |
|
