Figure 3. In situ hybridization for angiogenin in the human testis. Slide sections were hybridized with digoxigenin (DIG)-labeled antisense (B, C) or sense (A) riboprobes. Angiogenin messenger RNA (mRNA) is strongly expressed in testicular peritubular myoid cells, vascular endothelial cells, and smooth muscle cells. (D) shows hematoxylin-eosin staining of the same section, which shows the presence of mature spermatids in the seminiferous epithelium. Magnification: (A, B, D) 40x, (C) 100x. P indicates peritubular myoid cells; V, vascular endothelial cells. The section represents 6 samples stained with the same procedure.